Hordenine in Germinating Barley

Hordenine [4-(N,N-dimethylaminoethyl)phenol] is structurally related to tyramine, as well as several other compounds, such as catecholamines. It was earlier used in both human and veterinary medicine for the treatment of diarrhea and other intestinal disturbances. 

Hordenine was detected with a dual-electrode coulometric cell. The potential of the first electrode was set to -I-0.50V [vs. solid palladium reference electrode (Pd)], which was at the base of hordenine s hydro-dynamic voltammogram. This electrode cleaned the sample by removing easily oxidizable impurities. Hordenine and its precursors were subsequently detected by a second electrode at a potential of -I-0.75V. This method of detection is called the screen mode . In addition, the mobile phase was purified by connecting a coulometric cell between the pump and injector as a guard cell. This additional cell operated at +0.80 V. The detection limit of hordenine was at 1.1 ng, which was 25 times better than detection by UV at 275 nm. 

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