Hoppe-Seyler, Felix

At the time of Pasteur s quarrel with Berthelot over the publication of Bernard s posthumous manuscript, the number of the controversial soluble ferments approached 20, and in 1878 the German biochemist Willy Kuhne (1837-1900) suggested that such ferments should be called enzymes. At the same time, he carefully avoided taking sides in the conflict between vitalists and chemists, the new term was just a question of convenience. The name enzyme was not an immediate success. The leading German biochemist Felix Hoppe-Seyler (1825— 1895), for instance, said scornfully The new word enzyme could be added to the large number of new names that Kuhne has proposed for totally unknown substances. Nevertheless, with time the word enzyme would completely replace ferment and is now the only term used. 

Protamine sulfate is a strongly basic protein (white powder, see pK) used to precipitate nucleic acids from cmde protein extracts. It dissolves to the extent of 1.25% in H2O. It is freely soluble in hot H2O but separates as an oil on cooling. It has been purified by chromatography on an IRA-400 ion-exchange resin in the SO form and is washed with dilute H2SO4. Eluates are freeze-dried under high vacuum below 20°. This method is used to convert proteamine and protamine hydrochloride to the sulfate. [UV Rasmussen Hoppe Seyler s Z Physiol Chem 224 97 1934, Ando Sawada J Biochem (Tokyo) 49 252 1961, Felix Hashimoto Hoppe Seyler s Z Physiol Chem 330 205 1963.]... 

Felix, K., Zorn, K., and Dirr-Kaltenbach, H., Hoppe-Seyler s Z. physiol. Chem. 247,... 

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