Homogeneous Ferrocene-mediated Amperometric Immunoassay

Since binding of 4 to a specific antibody produces an electrochemicaUy inert entity, the intensity of the current is proportional to the quantity of free tracer in the medium, and thus inversely related to the quantity of analyte present. This is a competitive homogeneous immunoassay in which separation of the free and bound fractions of the tracer is not required (Fig. 8.12). 

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K. di Gleria, H.A. Hill, C.J. McNeil, and M.J. Green, Homogeneous ferrocene-mediated amperometric immunoassay. Anal. Chem. 58, 1203-1205 (1986). 

Gleiia KD, Hill HA, McneU CJ, Green Ml (1986) Homogeneous ferrocene-mediated amperometric immunoassay. Anal Chem 58 1203-1205... 

Fig. 8.12 Ferrocene-mediated amperometric homogeneous immunoassay of lidocaine. Only free ferrocenyl lidocaine tracer 4 is electrochemically active [28].

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