Homodecoupled Band-Selective HSQC

HQBS-HSQC Homodecoupled Band-Selective HSQC... 

Band-selective NMR spectroscopy experiments are really useful for peptides and proteins because a set of equivalent spins (amide NH, H , or aliphatic side-chain protons) appear in well-separated regions. Another alternative to obtain PS-HSQC spectra is by implementing the HOmodecoupled Band-Selective (HOBS) detection scheme [73,74]. This technique has been successfully implemented in a suite of NMR experiments involving IP H magnetization, such as Inversion-Recovery and CPMG-Project experiments... 

Figure 3 Pulse schemes to obtain pure shift HSQC spectra. (A) HSQC-RESET experiment which uses a pseudo-3D BIRD-based ZS acquisition scheme (B) PS-HSQC experiment using real-time homodecoupling by the combination of a hard 180°( H)-BIRD element during data acquisition (C) sensitivity-improved PS-HSQC (D) HOBS-HSQC experiment using real-time homodecoupling during detection achieved by applying a pair of hard/band-selective 180° pulses (represented as solid and shaded shapes). In (B-D), the homodecoupling element is applied at the middle of 2t=AQ//i periods, where AQ is the acquisition time and n is the number of concatenated loops. See original publications for a more detailed description.

---