Homochiral derivatizing agent

Derivatization with Optically Active Reagents and Separation on Achiral Columns. This method has been reviewed (65) a great number of homochiral derivatizing agents (HD A) are described together with many appHcations. An important group is the chloroformate HD As. The reaction of chloroformate HD As with racemic, amino-containing compounds yields carbamates, which are easily separated on conventional hplc columns, eg (66),... 

The reaction of a homochiral derivatizing agent with a heterochiral sample to yield covalently linked diastereoisomeric products is a precolumn technique used for the analysis of the individual enantiomers. The diastereoisomeric products are separable under a variety of chromatographic conditions (GC and LC), including both normal and reversed-phase procedures. Fig. 23 shows the LC separation of two diastereoisomeric products formed by the reaction of a heterochiral amine and a homochiral derivatization reagent. The derivatization reaction involves the formation of an amide by treating an A-substituted 5-prolyl chloride with racemic amphetamine (Fig. 24). 

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