Homocarnosine butyl derivative

Homocarnosine is a dipeptide of GABA and L-histidine. After deproteinizing the sample with ethanol, the mixtures are centrifuged. The clear supernatant is evaporated to dryness and derivatized with butanol. The sample is evaporated to dryness and redissolved in the mobile phase. The homocarnosine-butyl derivatives (Fig. 2.3.4) are quantified using liquid chromatography mass spectrometry/mass spectrometry (LC-MS/MS) operating in the positive mode. With multiple reaction monitoring (MRM), the transitions of m/z 297.0 to m/z 212.0 for homocarnosine and m/z 299.0 to m/z 212.0 for 2H2-L-homocarnosine are quantified. 

Fig. 2.3.4 Formation of the homocarnosine-butyl derivative from homocarnosine and l-butanol...

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