Homobifunctional reagents interactions

Figure 28.3 The homobifunctional crosslinkers BS2G and BS3 can be used to capture protein interactions through amide bond formation. The deuterium-labeled analogs of these reagents can be used to differentiate...

The use of PIR compounds to study protein interactions is a significant advance over the use of standard homobifunctional crosslinkers. The unique design of the PIR reagent facilitates deconvolution of putative protein interaction complexes through a simplified mass spec analysis. The software can ignore all irrelevant peak data and just focus analysis on the two labeled peptide peaks, which accompany the reporter signal of appropriate mass. This greatly simplifies the bioinformatics of data analysis and provides definitive conformation of protein-protein crosslinks. 

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