Hog renal acylase

A further chemical synthesis (93) (Scheme 22) relied on catalytic cis reduction of the (Z)-olefin 81, yielding the protected serine 82. This was resolved using hog renal acylase (EC 3.5.1.4), and BBrj treatment gave (2S, 3/ )-[2,3- H2]serine 60e contaminated with (2S)-[2,3,3- H3]serine. The (2S, 3S)-isomer could be obtained by a Mitsunobu reaction (93). 

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