Hippuryl-L-phenylalanine

The potential of the MALDI-MS-based assay scheme for the quantification of low molecular weight products and substrates directly from reaction mixtures has been described by Bungert et al. [8]. The glucose oxidase-based conversion of glucose to gluconolactone and the carboxypeptidase A-mediated cleavage of hippuryl-L-phenylalanine were chosen as model systems (Fig. 8.4). 

Fig. 8.4 (a) In the presence of oxygen, the glucose oxidase-catalyzed oxidation of y -D-glucose leads to the formation of gluconolactone. (b) Carboxypeptidase A selectively cleaves the substrate, hippuryl-L-phenylalanine, thus leading to the formation of hippuric acid and phenylalanine. 

Metal ion Peptidase activity Carbobenzoxyglycyl- L-phenylalanine Esterase activity Hippuryl-[Pg.180]

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