Hin recombinase

Hin recombinase FljB flagellin FljA protein (repressor) ... 

Regulation by Recombination In the phase variation system of Salmonella, what would happen to the cell if the Hin recombinase became more active and promoted recombination (DNA inversion) several times in each cell generation ... 

Two other proteins are required for efficient inversion by the Hin recombinase. A dimer of a 98-residue helix-turn-helix DNA binding protein called Fis (factor for inversion stimulation), a relative of protein HU,18b must bind to an enhancer, a 65-bp DNA segment. Binding of Fis to the enhancer helps to hold the supercoiled DNA and the recombinase in a correct orientation for reaction.576 595 596 Protein HU is also needed. The same Fis protein binds to an enhancer... 

Feng, J.-A., Johnson, R.C., Dickerson, R.E. (1994). Hin recombinase bound to DNA The origin of specificity in major and minor groove interactions. Science 263,348-355. 

A subtle (24°) base-pair opening of Ai3-Ti9 is induced by contacts of Hin recombinase (50) with the minor-groove edge of T19 (NDB entry pde009 Fig. 4c). By contrast, the major-groove capture of cytosine by Hhal DNA cytosine-5-methyltransferase... 

A schematic of a synthetic DNA-cleaving peptide bound to DNA that was constructed by synthesis of the DNA-binding domain of Hin Recombinase with Gly-Gly-His at the N-terminus to coordinate copper.Reproduced with permission from Reference 80. 

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