High-Throughput Sample Purification using MS-Detection

2 High-Throughput Sample Purification using MS-Detection 

At an early stage in the history of combinatorial synthesis it was apparent that product purity in excess of 80 % is not always attainable. In fact, purities of 30-40 % are quite common for several types of reaction. However, especially for single compounds from compound collections, high-throughput purification systems using HPLC have been developed. The degree of purity is first ascertained by analytical HPLC, and products having a purity below a pre-set threshold are subjected to this fully automated sample purification on a semipreparative or preparative scale. In first technical approaches, samples were 

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