High performance liquid cobalamins

High-performance liquid chromatography has, to date, only found limited applications in the analysis of sludges (aliphatic hydrocarbons, cationic and non-ionic surface active agents, carboxylic acids and cobalamin). [Pg.109]

DW Jacobsen, R Green, EV Quadro, YD Montejano. Rapid analysis of cobalamin coenzymes and related corrinoid analogs by high-performance liquid chromatography. Anal Biochem 120 394-403, 1982. [Pg.475]

Beck and Brink [232] have described a sensitive method for the routine assay of cobalamins in activated sewage sludge. The method involves extraction with benzyl alcohol, removal of interfering substances using a combination of gel filtration and chromatography on alumina, concentration of the extract by lyophilisation, and direct determination of total cobalamin by high performance liquid chromatography, in comparison with cobalamin standards. [Pg.129]

Determining the Snbtypes of Cobalamin. Based on differences in retention times between different forms of cobalamins, reversed-phase high-performance liquid chromatography (HPLC) has been used to separate the active subtypes of cobalamin and also to separate cobalamin from cobalamin analogues (Jacobsen et al. 1982). [Pg.462]

EP Frenkel, RL Kitchens, R Prough. High performance liquid chromatographic separation of cobalamins. J Chromatogr 174 393-400, 1979. [Pg.559]

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