High-performance liquid chromatography data presentation

The concept that molecular weight and polarity play a role in determining the composition of an asphaltene fraction is very realistic but does not present any indication of the nature of the polynuclear aromatic systems in the asphaltene fraction of petroleum. Thus, the purpose of this chapter is to present new data that provide indications of the ring-size distribution in petroleum asphaltenes. The evidence is accumulated by asphaltene fractionation and subsequent examination of the fractions by high-performance liquid chromatography (HPLC) using a UV detector. 

Elucidation of the structure of carotenoids requires, in addition to VIS/UV spectrophotometry, supplemental data from mass spectrometry and IR spectroscopy. Carotenoids are determined photometrically with high sensitivity based on their high molar absorbancy coefficients. This is often used for simultaneous qualitative and quantitative analysis. New separation methods based on high performance liquid chromatography have also proved advantageous for the qualitative and quantitative analysis of carotenoids present as a highly complex mixture in food. 

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