High-performance Liquid Chromatography and Related Techniques

High-performance Liquid Chromatography and Related Techniques 

CyDs have been used as major chiral mobile phase additives (CMPAs) for enantio-separations in HPLC. The first application of 8-CyD as a CMPA in combination with an achiral reversed-phase material for HPLC enantioseparations was reported by Sybilska and co-workers in 1982 [27]. These authors could achieve partial resolution of the enantiomers of mandelic acid and derivatives. The CMPA method played an important role in HPLC enantioseparations before the development of effective chiral stationary phases (CSPs) but is now rarely used. The major disadvantage of this technique, together with difficulties associated with the isolation of resolved enantiomers, is the rather large consumption of chiral selector. 

CyD-based columns for HPLC enantioseparations are well established and several tens of these columns are commercially available at present. Although highly suitable for analytical-scale enantioseparations these columns hardly compete with polysaccharide and macrocydic antibiotic-based CSPs for preparative-scale enantioseparations. More research is needed in this area. 

---