High-performance cation-exchange chromatography

Callmer, K. and Davies, L., Separation and determination of vitamin Bj, B2, Bj, and nicotinamide in commercial vitamin preparations using high-performance cation-exchange chromatography, Chromatographia, 7, 644, 1974. 

Mario N, Baudin B, Aussel C, Giboudeau J. Capillary isoelectric focussing and high-performance cation-exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants. Clin Chem 1997 43 2137-42. 

HPCEC, High-performance cation exchange chromatography IPAD, Integrated pulse amperometric detection ISWD,... 

The most appropriate way to solve mobile-phase incompatibility problems in LC-MS is to change the mobile-phase composition to LC-MS compatible conditions. This is the approach taken in most cases. Unfortrmately, it is not always possible to do so. In some cases, specialized LC colunrn materials demand a particular mobile-phase composition. This is the case with for instance some chiral colunms, which will only provide adequate enantiomeric separation in a predefined mobile phase. The retention behaviour in high-performance anion-exchange chromatography (HPAEC) is significantly influenced by the cation (sodium or ammonium) in the mobile phase. In these cases, mobile-phase... 

Tanaka and Ishizuka [3] studied the separation of acids by ion-exclusion chromatography on a modern, high performance cation-exchange column. Hitachi cation exchange resin, 8 % cross-linking, 18 2 pm particle size was used. Retention volumes of acids were obtained at 50 °C using water as the eluent. These are given in Table 8.1. 

Breter, H.-J., Seibert, G., and Zahn, R. K., Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations, ]. Chromatogr., 140, 251, 1977. 

Xu, W. and Regnier, F. E., Electrokinetically driven cation-exchange chromatography of proteins and its comparison with pressure-driven high-performance liquid chromatography, /. Chromatogr. A, 853, 243, 1999. 

Tanaka, K., Ohta, K., Haddad, P. R., Fritz, J. S., Miyanaga, A., Hu, W., Hasebe, K., Lee, K. P., and Sarzanini, C. (2001) High-performance Ion-exclusion/cation-exchange Chromatography of Anions and Cations in Acid Rain Waters on a Weakly Acidic Cation-exchange Resin, J. Chromatogr. A 920, 239-245. 

Figure 5 High-performance strong cation-exchange chromatography of ephedrine alkaloids and synephrine in dietary supplements. UV and fluorescence chromatograms of (A) standard solution ( 3 ng per component, except 1.6 tg of component 1), (B) Xenadrine RFA-1, (C) Xetalean, and (D) Ultra Diet Pep. Analytes 1, ( )-synephrine 2, (-)-norephedrine 3, (+)-norpseudoephedrine-HCI 4, (-)-ephedrine-HCI 5, (+)-pseudoephedrine 6, (-)-A/-methylephedrine 7, (+)-N-methylpseudoephedrine. Reproduced from R. A. Niemann M. L. Gay, J. Agric. Food Chem. 2003, 51, 5630-5638.

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