Heterologous gene expression Plasmid vectors

Further aspects Recently, improvements of the properties of plasmids have been made, such as the plasmid-based alpha-virus replicons [24]. These vectors lead to the production of replicon RNAs, which exit the nucleus, amplify themselves and express heterologous genes (e.g., antigens) to a high level. 

Mather, M.W. and Fee, J.A. (1992) Development of plasmid cloning vectors for Ihermus thermophilus HB8 expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene. Appl. Ertvirort. Microbiol, 58, 421-425. 

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