Heterogeneous Enzyme Kinetics

Andr Dianes , Roberto Femandez-Lafuente, Jose M. Guisan and Lorena Wilson  

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In order to give a homogeneous distribution of enzyme molecules inside the membrane, it was necessary to synthesize the membrane and to incorporate the enzymes at the same time. The co-cross-linking of enzyme molecules with an inert protein appears to be a proper solution. Purely active proteic films were created by using this procedure.11-12 These artificial enzyme membranes can be used in the study of heterogeneous enzyme kinetics and for modeling biological membranes. The phenomena in the enzyme membranes can be classified in two parts. 

Fig. 6.67 Classification of the methods for enzyme immobilization. (From A lllanes, R. Fernandez-Lafuente, J.M. Guisan, L Wilson, Heterogeneous enzyme kinetics, in A lllanes (Ed.), Enzyme Biocatalysis, Springer, 2008, pp. 155—203. Copyright 2008 Springer).

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