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Heterobifunctional reagents conjugation

Carlsson, J., Drevin, H., and Axen, R. (1978) Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3(2-pyridyldithio)propionate, a new heterobifunctional reagent. Biochem. J. 173, 723-737. [Pg.1052]

Antibody conjugates with GO can be made using the cross-linking agents glu-taraldehyde (Chapter 10, Section 1.2) or SMCC (Chapter 10, Section 1.1). The heterobifunctional reagent SMCC provides the best control over the conjugation process and usually results in high-activity preparations. [Pg.655]

IgG antibody may be labeled with HRP using glutaraldehyde in a two-step procedure (2) this produces small conjugates, but their activity is low. Heterobifunctional reagents such as succinimidyl 4-(7V-maleidomethyl)-cyclohexane-1 -carboxylate may also be used to link the thiol group of Fab fragments to an amino group in the enzyme (3). [Pg.70]

See other pages where Heterobifunctional reagents conjugation is mentioned: [Pg.642]    [Pg.32]    [Pg.35]    [Pg.35]    [Pg.35]    [Pg.276]    [Pg.277]    [Pg.283]    [Pg.295]    [Pg.296]    [Pg.300]    [Pg.324]    [Pg.768]    [Pg.788]    [Pg.850]    [Pg.875]    [Pg.897]    [Pg.964]    [Pg.965]    [Pg.965]    [Pg.967]    [Pg.993]    [Pg.113]    [Pg.115]    [Pg.81]    [Pg.44]    [Pg.44]    [Pg.44]    [Pg.46]    [Pg.248]    [Pg.249]    [Pg.265]    [Pg.267]    [Pg.272]    [Pg.280]    [Pg.481]    [Pg.539]    [Pg.588]    [Pg.653]    [Pg.654]    [Pg.656]    [Pg.683]    [Pg.1136]    [Pg.230]    [Pg.242]   
See also in sourсe #XX -- [ Pg.766 ]



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