Lespedezae herba

Crataegi folium Powdered drug (5g) is extracted with 75 ml ethanol (45%) for Ih under reflux. The Lespedezae herba filtrale is evaporated to approximately 20ml and transferred into a separation funnel (Procyanidines) 30 ml dichloromethane and 2 ml ethanol are added and shaken for 5 min, and the lower phase is discarded. Another 20 ml dichloromethane is added, and after shaking the lower phase is removed. This is repeated twice. The resulting extract is evaporated to approximately 10 ml. 

Crataegi folium, fructus, flos Lespedezae herba... 

Fig. I6A A methanoiic extract of Lespedezae herba (4) contains more highly glycosidated flavonoids (R, range 0.05-0.35) such as kaempferol- and/or isorhamnetin-rhamno-glucoside and rutin (Tl). The EtAc extract (5,6) contains the enriched yellow zones of flavon-C-glyco.sides such as isoorientin (R, -- 0.S/T3), orientin (R, — 0.65/T3) overlapped by isovitexin, a.s well as flavonol-O-glycosides such as lespedin, hyperoside, isoquerdtrin and querdtrin (Rf 0.6-0.8).

After treatment with the VP reagent, Lespedezae herba (5a. 6b) shows the phenolic compounds as red-brown zones (vis.). In addition to free catechin, epicatechin (R, -0,9), the dimeric procyanidins migrate into the upper R, range and trimeric procyanidins are found in the lower R, range. 

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