Heparin immobilised regioselective modified

Surface Concentration of Immobilised Regioselective Modified Heparin Derivatives... 

The regioselective modified heparin derivatives and heparin were immobilised on cellulose according to following scheme. 

Also a new end point attachment of nondegraded glycosaminoglycan type 2 (HE) or regioselective modified heparin derivatives will be described the reducing end is oxidised by electrolysis and the aldonic acid lactone formed reacts with amino groups of the polymer surface. Also coatings by ionic immobilisation (HE, ES-HS) will be described. 

Figure. I. 6 measure made immobilisation concepts for the attachment of GAG typ 2 (HE ES-HS) and regioselective modified heparin derivatives on polymer surfaces to get different coating thickness (in nm). ...

Immobilisation of GAG Type 2 (HE, ES-HS) and Regioselective Modified Heparin Derivatives... 

The results of surface coating 10 different polymers with HE res. ES-HS can be seen in Table 1. GAG type 2 and regioselective modified heparin derivative immobilisation was achieved by amide bond formation between carboxyl groups on the polymer surface and amino groups in the GAG chain or vice versa carboxyl groups were activated by the water soluble carbodiimide N-cyclohexyl-N -(2-morpholinoethyl)carbodiimide-methyl-p-toluene sulphonate (CME-CDl). 

Ionic immobilisation of heparin is a relative simple and rapid method for the preparation of short duration antithrombotic surfaces. Maximum anticoagulant activity of the immobilised molecules is obtained because of high flexibility of the chains. Because of relatively weak surface bonding, ionic immobilised HE, ES-HS and regioselective modified HE derivatives are not intended for long time applications. 

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