Iron absorption hemoglobin incorporation

In the hemoglobin incorporation method, absorption is estimated from the amoiint of tracer appearing in the blood. The principle of this method is based on the observation that absorbed iron is incorporated into circulating hemoglobin 10 to H days after dosing (7). A factor of 75% to 90% is usually introduced to correct for the incomplete utilization of absorbed iron for hemoglobin synthesis in normal subjects (8). 

The aim of this study was to further explore the potential and limitations of using stable iron isotopes as tracers and EI-MS in absorption studies. Procedures were developed for preparing iron acetylacetonate from both blood and fecal samples for mass spectrometric analysis. The precision and accuracy of ion abundance measurements were evaluated. In vivo use of stable iron isotope tracers was tested with a human study in which 54pe and 57pe were given orally and absorption was estimated with the fecal monitoring and hemoglobin incorporation methods. 

Table VII. Absorption of stable iron isotopes in orange juice measured with the fecal monitoring and hemoglobin incorporation...

Errors associated with iron quantification and abimdance measurements are common to both fecal monitoring and hemoglobin incorporation methods According to Janghorbani and Young (2 )> acceptable absorption estimates can be obtained if the precision of these measurements is kept below 5% The analytical procedures developed in this study are thus considered satisfactory since the relative standard deviation was 2 48% for total iron quantification and less than 2% for ion abimdance determinations ... 

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