Hemin-Aptamer DNAzyme-Based Aptasensor

Another type of widely used colorimetric aptasensor employs hemin and its aptamer as a DNAzyme complex. Since the DNAzyme complex catalyzes chemical reactions to produce colored products, which is different from the colorimetric detection described above, we will discuss this type of aptasensor in a separate section. 

Winner s group has been engaged in applying this method to DNA sensing and has made many improvements (Xiao et al., 2004 Yi et al., 2004). Usually, they have employed a peroxidase-like catalytic reaction in which hemin-aptamer complex catalyzes the reaction oxidation of 2,2 -azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) by H2O2 (Xiao et al., 2004). The product (ABTS+) is a colored molecule that can transfer signals directly by its absorbance at 414 mn. 

STRATEGY FOR USE OF SMART ROUTES TO PREPARE LABEL-FREE APTASENSORS 

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