Hematoxylin solution preparation

Convenient ready-to-use nuclear counterstains in blue, green or red colour like Hematoxylin, Methyl Green and Nuclear Fast Red are available from Vector Laboratories (http //www.vectorlabs.com/), DAKO (http //www.dako.com/) and some other vendors, but if you are interested in preparing nuclear counterstains yourself, good and thorough procedures may be found in textbooks of Polak and Van Noorden (1997) and Renshaw (2007) or on the website http //www.ihcworld. com/counterstain solutions.htm. 

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