Hematopoietic activity

The most immature cells remain in the adherent layer that is described as cobblestone areas, and are released to the growth media upon division and maturation. Major cell types in the cellular environment are macrophages, adipocytes cells and blanket cells. From the cobblestone areas hematopoietic cells are released into the growth media until the cultures begin to decline (8 weeks or later). Sign that the hematopoietic activity is declining is predominance of macrophages in the non-adherent cell population and decline of hematopoietic cell foci in the adherent layer (Dexter 1982). 

Curtis, B.M., Williams, D.E., Broxmeyer, H.E., Dunn, J., Farrah, T., Jeffery, E., Clevenger, W., de Roos, P., Martin, U., Friend, D., Craig, V., Gayle, R., Price, V., Cosman, D., March, C., and Park, L. (1991). Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein. Proc. Natl. Acad. Sci. USA 88, 5809 5813. 

GIO. Glass, G. B. J., Hematopoietic activity of glandular mucoprotein from human gastric juice. Gastroenterology 23, 219-228 (1953). 

L2. Landboe-Ghristensen, E., and Wandall, H. H., Hematopoietic activity (intrinsic factor effect) of pure pyloric secretion from the hog. Acta Med. Scand. 144, 467-472 (1953). 

Vitamin B 2 with intrinsic factor is a mixture whose potency is expressed in terms of oral units of hematopoietic activity. One unit has no more than 15 g of cyanocotelomin activity. The intrinsic factor is obtained from dried hog stomach. pylorus, or duodenum. One unit contains not more than 300 mg of dried product. 

In adults, RBCs are formed in the marrow of the vertebrae, ribs, sternum, clavicle, pelvic (iliac) crest, and the proximal epiphyses of the long bones. In children, most bone marrow space is hematopoietically active to meet increased RBC requirements. 

The product CARN 750, an injectable form of acemannan derived from Aloe barbadensis Miller, was administered for 18 d in subcutaneous doses of 0.5 mg, 1 mg, and 2 mg to irradiated mice. Hematopoietic activity of CARN... 

W., Faherty, D. A., and Kilian, P. L. Hematopoietic activities of interleukin-1 alpha In vitro and in vivo modulation by an anti-IL-1 receptor antibody. Prog. Clin. Biol. Res. 349,355-363... 

Joshima H, Kashima M, Enomoto H, et al. 1981. Effect of polymeric plutonium on the hematopoietic activity in the bone marrow of mice. J Radiat Res 22 425-433. 

Effect on Lymphopoietic Tissue. The changes in the hematopoietic tissue are the most striking morphological changes induced by glucocorticoid administration. The changes include destruction of the lymphoid tissue under acute administration of the hormone and stimulation of hematopoietic activity when the hormone s activity is consistently increased, as in Cushing s disease. 

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