Hemagglutination reaction inhibitor

Table XIII shows the potency with which AFGP inhibited hemagglutination by Osage orange (Maclura pomifera) lectin. At a final concentration in the reaction mixtures of less than 1 /xg/ml, the active AFGP completely inhibited hemagglutination of both human group-0 and sheep erythrocytes. By contrast, the inactive AFGP component was some 50-100 times less active as an inhibitor. Several other randomly tested preparations containing glycoproteins of diverse origin were either inactive or comparatively weak inhibitors.

It is still not understood how penicilloic acids could so rapidly produce in vivo the bi- or plurivalent antigens required for elicitation of anaphylactic reactions, unless the reaction rates of penicilloic acids with proteins in vivo are efficiently catalyzed (Levine and Ovary 1961 Schneider et al. 1973). Penicilloic acid may also act as inhibitor of reactions of BPO specificity. This has also been demonstrated in hemagglutination, precipitation, and guinea pig PCA (Josephson 1960 Torii and Horiuchi 1961 de Weck 1962a,b Levine 1963 Batchelor and Dewdney 1968). In general, penicilloic acid is a worse inhibitor than BPO amides but markedly more efficient than benzylpenicillin. 

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