Heat treatment temperature reaction kinetics affected

Denatured proteins often show irreversible behaviour at high temperature. A mathematical procedure for the analysis of heat capacity curves affected by irreversible first order unfolding was suggested by Sanchez-Ruiz et al. [12]. However, in that study the unfolding reaction was treated only as a kinetic phenomenon. Therefore no equilibrium parameters could be obtained. In a later paper [13] other treatments of the C,-curves were suggested which allowed for the extraction of equilibrium parameters from the non-equilibrium C -curve. 

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