Heat-Assisted Fixation with Osmium Tetroxide

MICROWAVE HEAT-ASSISTED FIXATION WITH OSMIUM TETROXIDE 

Standard chemical fixation fails to preserve extracellular materials. In contrast, Os04-microwave heating or the high-pressure freezing-freeze-substitution technique is able to preserve such materials (Eggli and Graber, 1994). The former technique is simpler 

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