Haptenated basic polymers

Fig. 7.21. Enzyme coupling via p-benzoquinone (pBQ) activation (pBQ also reacts with carbohydrates) and coupling to a basic polymer (I). At pH 6, only one of two potential sites of pBQ reacts, whereas at pH 8 the second site is activated. The complex is conjugated to denatured DNA using glutaraldehyde. Alternatively, haptenated basic polymers can be linked by 1,2,7,8-diepoxyoctane (carcinogenic ) to DNA (II).

Crosslinking of haptenated or enzyme-labeled basic polymers... 

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