Hapten—carrier conjugation cross-linkers

Succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (SMCC) is a heterobifunctional cross-linker with significant utility in cross-linking proteins, particularly in the preparation of antibody-enzyme and hapten-carrier conjugates (Hashida and Ishikawa, 1985 Dewey et al., 1987). The NHS ester end of the reagent can react with primary amine groups on proteins to form stable amide bonds. The maleimide end of SMCC is specific for coupling to sulfhydryls when the reaction pH is in the range of 6.5-7.5 (Smyth et al., 1964) (Fig. 158). 

The following is a generalized protocol for the activation of a protein with sulfo-SMCC with subsequent conjugation to a sulfhydryl-containing second molecule or protein. Specific examples of the use of this cross-linker to make antibody—enzyme or hapten—carrier conjugates may be found in Chapter 10, Section 1.1, and Chapter 9, Section 5, respectively. 

NHS Ester-Maleimide Heterobifunctional Cross-linker-Mediated Hapten-Carrier Conjugation... 

Figure 283 Hapten-carrier immunogen conjugates can be formed using homobifunctional NHS ester cross-linkers. The reaction may create large polymeric complexes, some of which could precipitate.

SMCC is a heterobifunctional cross-linker with significant utility in cross-linking proteins, particularly in the preparation of antibody—enzyme (Chapter 10) and hapten-carrier (Chapter 9) conjugates (Hashida and lshikawa, 1985 Dewey et al.,... 

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