H protein

Generic name R CAS Registry Number SolubiHty in water, 25°C, mg/100 ml. Lip 0 solubiHty, %" Human plasma half-life", h Protein binding % bound... 

Chen H, Zhang Z, Chen Y, Brook MA, and Sheardown H. Protein repeUant silicone surfaces by covalent immobilization of poly(ethylene oxide). Biomaterials, 2005, 26, 2391-2399. 

Figure 2 Immobilized antigen ELISA format. Antigen is immobilized to a solid phase by passive adsorption. Following removal of unbound antigen, analyte (free H) and antigen (H-protein) compete for a fixed number of primary antibody (Y) binding sites. Unbound materials are removed (dotted line). Secondary antibody-enzyme conjugate (Y-E) is added to bind to primary antibody followed by another wash step. Substrate (A) for the enzyme is added to detect the bound enzyme. The amount of colored product ( ) detected is inversely proportional to the amount of analyte present...

Reitsma P. H. Protein C deficiency From gene defects to disease. Thromb Haemost 1997 78, 344-50. 

Milligan, G. and White, J. H., Protein-protein interactions at G-protein-coupled receptors, Trends Pharmacol. Sci., 22(10), 513-518, 2001. 

Hubbard, S. R. and Till, J. H., Protein tyrosine kinase structure and function, Annu. Rev. Biochem., 69,373-398, 2000. 

Measles virus hemagglutinin (H) protein Carrot leaf and root IgGl and IgG2 cross reacted strongly with measles virus and neutralized virus in vivo. Immunogenic in mice when delivered parenterally. 39... 

Rinderpest virus hemagglutinin (H) protein Pigeon pea leaf 110... 

Schulman, H,. Protein phosphorylation in neuronal plasticity and gene expression. Curr. Opin. Neurobiol. 5 375-381,1995. 

El wing, H., Protein adsorption and ellipsometry in biomaterial research, Biomaterials 1998, 19,397 406... 

S. H., Protein S-nitrosylation a physiological signal for neuronal nitric oxide, Nat. Cell Biol. (2001), p.3193-3197... 

Fig. 1. (opposite page) Distribution of FITC-conjugated BSA in various fibroblast cell lines under different fixation/permeabilization regimes. (A-D) Protein distribution in living cells (A) PtKj, (B) CHO, (C) 3T3, and (D) HeLa cells. The protein is excluded from the nuclei of all cells. (E-H) Protein distribution in cells extracted for 10 min with 0.1% Triton X-100 before fixation for 30 min with 3.7% formaldehyde (E) PtKi, (F) CHO, (G) 3T3, and (H) HeLa cells. Nuclear fluorescence is seen in (E) PtKj and (G) 3T3 cells. (I-L) Protein distribution in cells extracted for 10 min with 1% Triton X-100 before fixation for 30 min with 3.7% formaldehyde (I) PtKj, (J) CHO, (K) 3T3, and (L) HeLa cells. No fluorescence is detected in the cells with the exception of some nuclear fluorescence seen in (L) HeLa cells. (M-P) Protein distribution in cells fixed for 30 min with 3.7% paraformaldehyde before permeabilization for 10 min with 0.1% Triton X-100. Fluorescence is seen primarily in the cytoplasm with the exception that nuclear fluorescence is seen in (M) PtKi and (N) CHO cells. (Q-T) Protein distributions in cells fixed for 5 min with 90% methanol, 50 vaM EGTA at -20°C (Q) PtKj, (R) CHO, (S) 3T3, and (T) HeLa cells. All cells show an overall low fluorescence, fibrous-textured cytoplasmic fluorescence, and bright staining at the periphery of the nucleus. 10 mm per scale division (black bar). (Reproduced with permission from ref. 6.)... 

Kinoshita K, Nakamura H. Protein informatics towards fimction identification. Curr Opin Struct Biol 2003 13 396 00. 

Koch MA, Waldmann H, Protein structure similarity clustering and natural product structure as guiding principles in drug discovery. Drug Discov Today... 

Antiarrhythmics Onset (h) (oral) Duration (h) Half-life (h) Protein binding (%) Excreted unchanged (%) Therapeutic serum level (mcg/mL) Toxic serum levels (mcg/mL)... 

SSRis Time to peak piasma concentration (h) Haif-iife (h) Protein binding (%) Time to reach steady state (days) Primary route of eiimination... 

Lindmark, R., Biriell, C., and Sjoquist, J., Quantitation of specific IgG antibodies in rabbits by a solid-phase radioimmunoassay with H-protein A from Staphylococcus aureus, Scand. J. Immunol., 14, 409 20, 1981. 

Takeuchi T, Goto D, Shinmori H. Protein profiling by protein imprinted polymer array. Analyst 2007 132 101-103. 

The quinolones have long enjoyed a favorable pharmacokinetic profile. They are well absorbed and distributed in body tissues and fluids. A typical dose is between 100 mg and 1 g. The older agents have half-lives under 3 h, but a more typical value is between 4 and 14 h. Protein binding tends to be low to moderate (15-65%), but there are some exceptions such as nahdixic acid (90%) and garenoxacin (80%) (Howe and MacGowan, 2004). Bioavailability ranges from 55 to 100% (Dudley, 2003b). It has been well estabhshed in the hterature that the effectiveness of quinolones can be dramatically reduced if the medication is taken with an antacid. Many antacids are salts of divalent and trivalent cations such as Al " , Ca, and Mg " ". In addition, Fe " , Cu " , Ni " ", Zn " ", and also reduce quinolone activity. All these cations form a chelate with... 

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