Growth Effects with Johnes Bacillus

Naphthoquinones 1. Growth Effects with Johne s Bacillus 

Partially purified concentrates from cells of Mycobact. phlei (acetone extraction— ether- water) added at a level equivalent to 1% M. phlei cells, and thus supplying 200 mE- of solids/20 ml. medium, gave excellent growth. It was clear that, although the two naphthoquinones made an important contribution to the nutrition of the organism, the M. phlei extracts contained additional growth stimulants. 

Phthiocol was isolated from tubercle bacilli (269a) and since the Johne factor was soluble in fat solvents and water, Woolley and McCarter tested phthiocol for activity. The vitamin K activity of phthiocol suggested a test of other vitamin-K-active substances, and a highly potent concentrate of vitamin K, and also the simple 2-methyl-l, 4-naphthoquinone were thus found to be active for Johne s bacillus. 

Thus vitamin-K-active substances, of which phthiocol is a natural 

Naphthoquinones and the Antagonism of lodinin Inhibition of Bacterial Growth 

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