GroEL fluorescence binding experiments

Figure 7.36 Peptide association with GroEL (a) Fluorescence emission spectra from fluorescence binding titration experiment with fixed concentration of AMPH+ peptide(0.5 / M) titrated with GroEL until saturation in buffer pH 7.5 at 20°C. Note blue shift and increase in emission intensity as titration progresses. (b) Three binding isotherms from three fluorescence binding titration experiments involving the indicated peptides under conditions described in (a) (Reproduced from Preuss et al., 1999, Fig. 6).

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