Gradient separations constant slope

Figure 12 Chromatographic resolution and analysis time for mobile phase gradients designed to produce different average capacity factor. Amino acid separations using an average capacity factor (k" of 23 (A), 11.6 (B), and 5.8 (C). The gradient amplitude (35-63%B), flow rate (0.3 pL/min), and injection volume (250 nL) for all the separations were held constant while the gradient slope was varied. Anal5d e and retention order the same as in Figure 9.

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