Gradient HSQC-TOCSY

Gradient enhanced versions of the ID HMQC-TOCSY and ID HSQC-TOCSY have also been published recently [31, 55]. 

Load the spectrum of the gradient-assisted, inverse detected, 2D CH-HSQC-TOCSY experiment acquired with the echo-antiecho technique, D NMRDATA GLUCOSE 2D CH GCHICOTO 001999.RR. Check and if necessary correct its calibration in both dimensions. Set up a layout as for the basic HSQC spectrum. Compare the spectrum with the spectra of the basic HSQC and HMQC experiments. Use the same rows or columns to identify the additional TOCSY-peaks. 

The assignment of the carbohydrate chains of an intact glycoprotein (de Beer et al., 1994) with the help of a gradient-selected natural abundance HSQC-TOCSY spectrum is a recent example of the use of Hartmann-Hahn-type experiments in the assignment of oligosaccharides (Dabrowski, 1994). 

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