Gradient elution conformational effects

Problems Protein sample and elution buffer must not contain any salt. However, some proteins need a certain salt concentration for the preservation of their native conformation. With membrane proteins or with level pH gradients, the focusing effect is weak and the sample is diluted. 

I In a second paper (75b) Heam et al. report band width data for 9 addi-9, Ikmal peptides in levcnrsed-phase gradient elution. With one exception, these band width data are in good agreement (/ w 0.2) with v ues calcu-> lat by our model, for values of b less than 0.5 (again the J effect was not takm into account). The wider bands found for one peptide were attributed to conformational effects. 

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