Golgins preparation

J., Satch, A., Pelletier, L., and Warren, G., Golgin tethers define subpopulations of COPI vesicles. Science 307,1095-1098,2005 Short, B Haas, A., and Barr, F.A., Golgins and GTPases, giving identity and structure to the Golgi apparatus, Biochim. Biophys. Acta 1744, 383-395, 2005 Satoh, A., Beard, M., and Warren, G., Preparation and characterization of recombinant golgin tethers. Methods Enzymol. 404, 279-296, 2005. 

Note that uncoating the COPI vesicles is required for binding to golgin tethers (or Golgi membranes, see below) since binding was not observed when an ArflQVlL mutant was used for preparation of COPI vesicles (Malsam et al, 2005). 

Satoh, A., Beard, M. B., and Warren, G. (2005). Preparation and characterization of recombinant golgin tethers. Methods in Enzymology 404, 279-296. 

Golgins are minor components of Golgi membranes so that preparation of sufficient amounts for biochemical analysis is difficult. Here we describe the preparation of recombinant forms of those golgins that constitute the tethering complexes just described. 

PREPARATION AND CHARACTERIZATION OE RECOMBINANT GOLGIN TETHERS 295 Acknowledgment... 

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