Oligosaccharides glycosyltransferases

Previous enzyme Certain glycosyltransferases will only act on an oligosaccharide chain If It has already been acted upon by another processing enzyme. ... 

Appropriate nucleotide sugars and highly specific Golgi-located glycosyltransferases are employed to synthesize the oligosaccharide chains of GAGs. The one enzyme, one linkage relationship appears to hold here,... 

Trincone, A. and Giordano, A. (2006) Glycosyl hydrolases and glycosyltransferases in the synthesis of oligosaccharides. Current Organic Chemistry, 10, 1163-1193. 

Rabbani, S., Schwardt, O. and Ernst, B. (2006) Glycosyltransferases an efficient tool for the enzymatic synthesis of oligosaccharides and derivatives as well as mimetics thereof. Chimia, 60, 23-27. 

R. Ohrlein, Glycosyltransferase-Catalyzed Synthesis of Non-Natural Oligosaccharides, in Biocatalysis—From Discovery to Application, ed. W. D. Fessner, Topics in Current Chemistry 200, Springer-Verlag, Berlin/Heidelberg, 1999, pp. 227. 

Ohrlein, R., Glycosyltransferase-catalysed synthesis of non-natural oligosaccharides. Topics Curr. Chem., 1999, 200, 227-254. 

In summary, different strategies for the combinatorial biosynthesis of oHgosaccharides have been established successfully. However, some systems are Hmited mostly to bacterial glycosyltransferases because of their better expression in E. coli. The yeast systems seem to be better suited for expression of mammahan glycosyltransferases however, it remains to be demonstrated if they can be also utilized as living factories for the production of oligosaccharides. 

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