Glycosylation enzyme-mediated

Human a-galactosidase is a 100 kDa homodimeric glycoprotein. Each 398 amino acid monomer displays a molecular mass of 45.3 kDa (excluding the glycocomponent) and is glycosylated at three positions (asparagines 108, 161 and 184). After administration (usually every second week by a 40 min infusion), the enzyme is taken up by various body cell types and directed to the lysosomes. This cellular uptake and delivery process appear to be mediated by mannose-... 

Enzymes are sometimes inactive as simple polypeptides. Addition of another group forms the fully active enzyme. Phosphorylation or glycosylation of an OH or NH in an R-group is typical. Phosphorylations are especially common, and this process is itself mediated by another enzyme, called a kinase. Kinases are an important family of enzymes, and kinase inhibition can be a powerful means of impacting cellular function. 

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