Glycosyl analytical applications

Supercritical fluid extraction (SEE) is another modern separation technology usually employed to extract lipophilic compounds such as cranberry seed oil, lycopene, coumarins, and other seed oils. Anthocyanins generally and glycosylated anthocyanins in particular were considered unsuitable for SEE due to their hydrophilic properties, since SEE is applicable for non-polar analytes. However, a small amount of methanol was applied as co-solvent to increase CO2 polarity in anthocyanin extraction from grape pomace. New applications of SEE for anthocyanin purification have been reported for cosmetic applications from red fruits. ... 

First accounts of the condensation of hydroxylamines with sugars can be found in the 1880s [39]. Owing to the lack of analytical methods to determine molecular structures, these early condensations were used for the characterization of sugars, the best known example of this chemistry is the Wohl degradation [40]. Here, we cover the more recent applications and characterization of O-aUcyl-N-glycosyl oxyamines. 

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