Glycoproteins to Amine-Dendrimers by Reductive Amination

Coupling Glycoproteins to Amine-Dendrimers by Reductive Animation 

The amines on the surface of PAMAM-type and other amine-containing dendrimers may be used to couple to aldehyde groups in other molecules, including those formed after periodate 

The following protocol involves the conjugation to an amine-dendrimer of a periodate-xidized glycoprotein, such as an antibody, which has been treated to produce aldehydes according to the protocols in Chapter 1, Section 4.4. This type of conjugation reaction 

Conjugation through Schiff base formation followed by reduction to secondary amine linkage. 

React for 2 hours at room temperature with mixing. 

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