Glycoproteins biocytin-hydrazide

The reactivity and use of biocytin-hydrazide is similar to that described for biotin-hydrazide in Section 3, this chapter. The following protocol for labeling glycoproteins at oxidized carbohydrate (galactose) sites is from Bayer and Wilchek (1992). 

To each ml of glycoprotein solution, add 30 pi of neuraminidase (1 unit/ml as supplied by Behringwerke AF), then 30 pi of galactose oxidase (previously dissolved at 100 units/ml in the labeling buffer of step 1), and finally 100 pi of the biocytin hydrazide solution. 

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