Glycopeptides preparative liquid chromatography

Fig. 4.3. High performance liquid chromatography (HPLC) of the monosaccharides obtained from a partially purified preparation of microbubble glycopeptide surfactant from forest soil. Following hydrolysis (in 2 N HC1 for 6 hr at 100°C) and filtration, the carbohydrate mixture was charged on a Bio-Rad HPX-87 cation exchange column. For comparison, part A shows the chromatogram (using the same HPLC column) of a standard solution, which contained 4 pg of each of three different monosaccharides (i.e., the last three peaks shown are glucose, xylose and fiicose, in the order of increasing retention times). Part B shows the chromatogram obtained from hydrolysis of the partially purified (see text) microbubble surfactant (approximately 30 pg). All other experimental conditions were identical in the two cases, i.e., water eluent, 0.5 ml/min flow rate, 85°C, refractive index detector attenuation -2x. (Taken from ref. 322.)...

$Fig. 4.3. High performance liquid chromatography (HPLC) of the monosaccharides obtained from a partially purified preparation of microbubble glycopeptide surfactant from forest soil. Following hydrolysis (in 2 N HC1 for 6 hr at 100°C) and filtration, the carbohydrate mixture was charged on a Bio-Rad HPX-87 cation exchange column. For comparison, part A shows the chromatogram (using the same HPLC column) of a standard solution, which contained 4 pg of each of three different monosaccharides (i.e., the last three peaks shown are glucose, xylose and fiicose, in the order of increasing retention times). Part B shows the chromatogram obtained from hydrolysis of the partially purified (see text) microbubble surfactant (approximately 30 pg). All other experimental conditions were identical in the two cases, i.e., water eluent, 0.5 ml/min flow rate, 85°C, refractive index detector attenuation -2x. (Taken from ref. 322.)...$

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