Glycerolipids mass spectrometry

Cool the tubes and add 75 (jlI of 1M KOH in methanol, sonicate and incubate for 2 hr at 37°C. This step removes most of the interfering glycerolipids, in particular phosphatidylcholines, that can mask sphingomyelins in a simple Mass spectrometry scan. 

A Valeur, P Michelsen, G Odham. On-line straight phase liquid chromatography/plasmaspray tandem mass spectrometry of glycerolipids. Lipids 28 255-259, 1993. 

Identifications of [M - H] ions of molecular species are given according to the selected m/z range. Data in range nVz 745-780 are shown. Molecular species are identified by number of acyl carbonsmumber of double bonds, p, alkenylacyl a, alkylacyl , disabled. (Reproduced with permission from Kurvinen J-P, Aaltonen J, Kuksis A, and Kallio H (2002) Software algorithm for automatic interpretation of mass spectra of glycerolipids. flap/d Communications in Mass Spectrometry 16 1812-1820.)... 

Kamido, H., Kuksis, A., Marai, L. et al. (1992b) Preparation, chromatography and mass spectrometry of cholesteryl ester and glycerolipid-bound aldehydes. Lipids, 27, 645-50. 

Kuksis, A., Myher, J. J., Yang, L.-Y. and Steiner, G. (1994) Glycerolipid metabolism with deuterated tracers, in Biological Mass Spectrometry Present and Future (eds T. Matsuo, R. M. Caprioli, M. L. Gross and Y. Sayama), John Wiley, Chichester, Sussex pp. 481-93. 

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