Glutaraldehyde endoplasmic reticulum fixative

Fig. 65. Prominent endoplasmic reticulum and ovoid electron-dense secretory granules in the apical cap of a type 4 Clara cell from a bronchiole (block 465) of a male rat (No. 5/104 E) which inhaled 71.5 mg trimethylol melamine (Madurit) dust/1 for a total of 32 h 30 min on 4 consecutive days. Fixed under pentobarbital anaesthesia (30 mg/kg) by intratracheal instillation of 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4) before opening the thorax. Postfixation with 1 % osmium tetroxide in sodium cacodylate buffer. Embedded in Epon 812 and sectioned at 50 nm. Lead citrate and uranyl acetate. Plate 4050...

Fig. 74. Smooth endoplasmic reticulum in the apical part of a types Clara cell from a female white rat (breeder Winkel-mann, Borchen-Kirchborchen) 2 h after an inhalation of 200 mg micronized isoprenaline HCl -1- 300 /ig micronized (mass median diameter 2.45 fjm) phenylephrine bitartrate/ uff from a suspension type self-propelled aerosol (Mediha-er). 12 Puffs/min were dispersed into a 164.5 1 box where the animals stayed for 15 min. 30 min later under methitural anaesthesia, the lung was fixed by intratracheal instillation of 2.5 % glutaraldehyde in phosphate buffer (pH 7.4) before opening the thorax. Postfixation with 1 % osmium tetroxide in phosphate buffer (pH 7.4). Contrasted en bloc for 12 h with 0.5 % uranyl acetate in 70 % ethanol. Embedded in a 2 8 mixture of methyl and butyl methacrylate. Sectioned at 50 nm. Lead citrate after Reynolds (1963). Film 374/84...

Terasaki, M. Song, J. Wong, J. R. Weiss, M. J. Chen, L. B. Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell 1984, 38, 101-108. 

---