Glutaraldehyde cross-linking procedure

Figure 302 Glutaraldehyde antibody—enzyme cross-linking procedures usually produce a wide range of high-molecular-weight complexes, some of which may precipitate from solution.

Enzyme membrane patterning procedures were utilized similar to the monofunctional FET biosensors, except that the membranes were developed in water instead of a glutaraldehyde solution. The center FET element was used as a reference FET employing a bovine serum albumini-immobilized membrane. After the three membranes were deposited on the FET surface, they were cross-linked by immersing them in a glutaraldehyde solution to make them mechanically strong. 

The final immobilisation procedure that have been used is cross-linking and involves joining the enzymes to each other forming a large, three-dimensional complex structure, achieved by physical or chemical methods. The chemical methods of cross-linking involve covalent bond formation between the protein molecules by means of a bi- multifunctional res ent such as glutaraldehyde and toluene diisocyanate. ... 

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