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Glutamine consumption, Specific rate

Typical kinetic profiles (hybridoma). (A) Cell concentration and viability (B) glucose consumption (GLC) and lactate production (LAC) (C) monoclonal antibody production (mAb) (D) glutamine consumption (GLN) and ammonium production (NH4+) (E) specific growth rate (px) (F) alanine (ALA) and glycine (GLY) production. Adapted from Lee (2003). Symbols correspond to the experimental data and the lines to the manual curve fitting. Vertical lines indicate the instant at which exponential growth phase ended (gx < Px.max)-... [Pg.184]

The specific rate of glutamine consumption (which takes into account glutamine decomposition) ... [Pg.166]

Specific glutamine consumption rate (mmol 10 cells h" )... [Pg.177]

Figure 27. Heat flux is plotted as a function of the specific growth rate ( ) showing the monotonically decreasing relationship. This dependence extends to the fluxes for lO" x IFN-y production flux, lU s per cell, (A) and the major catabolites, 10 x glucose consumption flux, mol s per cell, ( ) and lO x glutamine consumption flux, mol s per cell (A) (Reproduced from Reference [22] with permission). Figure 27. Heat flux is plotted as a function of the specific growth rate ( ) showing the monotonically decreasing relationship. This dependence extends to the fluxes for lO" x IFN-y production flux, lU s per cell, (A) and the major catabolites, 10 x glucose consumption flux, mol s per cell, ( ) and lO x glutamine consumption flux, mol s per cell (A) (Reproduced from Reference [22] with permission).
Optimal conditions for insect cell growth have been extensively studied, but for product expression with a baculovirus infected insect cell the focus should be on the difference in the metabolic requirements of infected vs. uninfected cells, which has been observed to differ after infection. The alanine specific production rate decreases almost four-fold, while phenylalanine specific consumption rate increase 11-fold and glutamine specific consumption decrease [65]. Both an increase [66] and a decrease [67] in glucose consumption rates of insect cells after infection have been reported. This reflects some differences in the media and vectors that were used however, it is normal to expect a higher metabolic burden after infection due to the increase in protein expression rates caused by the infection. This creates a concern about the impact of nutrient limitations on the productivity of the system. [Pg.194]

Figure 4.3.2 Evolution with time of the specific growth and death rates (a), the specific consumption rates of glucose (b) and glutamine (c) and the specific production rates of lactate (b), ammonia (c) and monoclonal antibodies (d) in VO 208 batch culture (data from Figure 4.3.1). Figure 4.3.2 Evolution with time of the specific growth and death rates (a), the specific consumption rates of glucose (b) and glutamine (c) and the specific production rates of lactate (b), ammonia (c) and monoclonal antibodies (d) in VO 208 batch culture (data from Figure 4.3.1).
Figure 4.3.3 Relationships between specific glucose (a) and glutamine (c) consumption rates, lactate (b), ammonia (d) and monoclonal antibody (e) production rates and specific growth rate during lag and growth phases of VO 208 batch culture. Figure 4.3.3 Relationships between specific glucose (a) and glutamine (c) consumption rates, lactate (b), ammonia (d) and monoclonal antibody (e) production rates and specific growth rate during lag and growth phases of VO 208 batch culture.

See other pages where Glutamine consumption, Specific rate is mentioned: [Pg.85]    [Pg.94]    [Pg.161]    [Pg.137]    [Pg.76]    [Pg.101]    [Pg.189]    [Pg.133]    [Pg.191]    [Pg.147]    [Pg.1048]    [Pg.172]   
See also in sourсe #XX -- [ Pg.161 , Pg.166 , Pg.170 , Pg.172 ]




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