Polymerization, glutamate dehydrogenase

Since glutamate dehydrogenase polymerizes, a correction for light scattering should always be made. 

Consider first three "homo-polymeric" enzymes, all shown to involve allosteric effects malate dehydrogenase, 3-phosphoglycerate dehydrogenase, and glutamate dehydrogenase. Malate dehydrogenase (Fig. 9.8), a dimer, has a single interface (29) 3-phospho-... 

The most intensively studied strictly allosteric effect of GTP is its inhibition of the catabolic enzyme glutamate dehydrogenase from animals (76-81) and lower eukaryotes (82, 83). The animal enzyme is inhibited by micromolar concentrations of GTP and is stimulated by ADP (76-81). ATP also inhibits but only at much higher concentrations than required by GTP. GTP acts in part by regulating the level of polymerization of the enzyme subunits. 

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