8-Glucosidases molecular weight

Different types of gel materials, such as polysaccharides, proteins and synthetic polymers, are now used to entrap biocatalysts. Among them, photo-crosslinkable resin prepolymer ENTP-4000 as shown in Eig. 7 is more useful compared to others. Entrapment of biocatalysts should be carried out under the illumination of near ultraviolet hght within 3-5 min, by which high temperatures, shifts of pH to extremely alkahne or acidic sides are avoided. ENTP-4000, hydrophobic photo-crosslinkable resin prepolymer, is one of the most suitable prepolymers for entrapment of p-glucosidase. Molecular weight of its main chain is about 4000. 

Table IV shows the biochemical features of thomostable a-glucosidase purified 140-fold from C. thermohydrosulfuricum (Saha and Zeikus, unpublished work). The enzyme has a 162,000 molecular weight and displayed an optimum temperature for activity of 75°C. Notably, the protein preparation hydrolyzed both a-1,6 and a-1,4 linkages. This enzyme appears to play an important role in starch degradation by C. thermohydrosulfuricum because it can hydrolyze the degradation intermediates formed by the organism s unique amylopullulanase.

The glucosidases involved in the biosynthetic pathway have been studied in detail by Hemscheidt and Zenk 203), and two of the isolated enzymes were specific for the hydrolysis of strictosidine (33) and were purified 120-fold. The enzyme was isolated from a number of indole alkaloid producing plants, including C. roseus, C.pusilus, and C. trichophyllus. The pH optimum was 6.5, and the values were 0.2 mM for enzyme I and 0.1 mM for enzyme II. Molecular weights were estimated at 230,000 for enzyme I and 450,000 for enzyme II. Unlike the case of the enzyme system of Scott et al. 198,199), tryptamine did not activate either of the two enzymes. The enzymes were highly substrate specific vincoside (85)... 

D-Glucosidases Estimates of Molecular Weight and Molecules per Yeast Cell... 

The /3-D-glucosidases studied were intracellular (cytoplasmic) enzymes of high molecular weight, about 300,000 (see Table XII), but... 

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