Glucose tritium-labeled, preparation

A synthesis of hexa-acetyl streptamine (14) from 2-acetamido-2-deoxy-D-glucose is outlined in Scheme 5. The tritium-labelled 2,6-dideoxystreptamine (15) has been prepared from a trihydroxycyclohexanone derivative by a sequence of standard reactions that included the reduction of the ketone group with sodium borotritide. ... 

A control diet was prepared as follows Tha labelled egg-white protein was mixed with a basal diet in the proportion 2 98. The basal diet contained 10% casein, 10% sucrose, about 70% starch and 5% maizeoil, as well as vitamins and minerals. A small amount of [sHl-lysine was added to this mixture, giving a ratio of tritium to (11 C) dpm of approximately 10 1. The experimental diet was obtained by adding a small amount ot the glucose-lysine reaction mixture to the control diet. 

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