Glucose oxidase/lactoperoxidase reaction

Fig. 4. Elution pattern of I-labeled rat prolactin, [ I]rPRL (AFP 1-2), prepared by the glucose oxidase-lactoperoxidase iodination method and purified as described in Fig. 1 for human PRL. Before this material was labeled, it had been diluted in phosphate buffer to 100 /xg/ml, aliquoted into separate reaction vials, and stored at -20° for 1 month. During storage, the unlabeled hormone apparently converted to aggregate, as the talc-resin-(TCA) test indicated that no usable monomeric hormone remained available for iodination (cf. Fig. 3) (Tower et al. ).

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